Document 0648
 DOCN  M9480648
 TI    Diagnostic value for culture of cerebrospinal fluid from HIV-1-infected
       individuals for opportunistic viruses: a prospective study.
 DT    9410
 AU    Dix RD; McCarthy M; Berger JR; Department of Ophthalmology, University
       of Miami School of; Medicine, Florida.
 SO    AIDS. 1994 Mar;8(3):307-12. Unique Identifier : AIDSLINE MED/94304550
 AB    OBJECTIVE: To investigate the diagnostic value of cerebrospinal fluid
       (CSF) culture for opportunistic viruses from HIV-1-infected individuals.
       METHODS: A 4-year prospective study was conducted using a participant
       population consisting of 186 HIV-1-infected individuals without
       neurologic disease, 73 HIV-1-infected individuals with encephalopathy,
       myelopathy, and/or peripheral neuropathy, and 10 controls. CSF samples
       recovered at 1-year intervals were subjected to virus culture using
       technique commonly used in the clinical laboratory setting. RESULTS: CSF
       samples obtained from only 15 of the 269 (5.6%) participants yielded an
       opportunistic virus upon culture. Cytomegalovirus, herpes simplex virus
       types 1 and 2, adenovirus, and presumptive enteroviruses were
       identified. No consistent correlation was observed between the detection
       of an opportunistic virus within a CSF sample and the presence or future
       development of neurologic disease. However, a significant correlation
       was observed between culture of virus from CSF and the future
       development of abnormal CD4+ (chi 2, P = 0.0286) and CD8+ (chi 2, P =
       0.0018) lymphocyte counts in HIV-1-infected participants without
       neurologic disease. CONCLUSION: These results show that culture of CSF
       to screen for opportunistic viruses is neither diagnostic nor predictive
       of neurologic disease in HIV-1-infected individuals. Nevertheless, the
       presence of virus within CSF may be an indicator of HIV-1-mediated
       immune dysfunction and a predictor for future development of abnormal
       CD4+ and/or CD8+ lymphocyte counts.
 DE    Antigens, CD8  AIDS-Related Opportunistic
       Infections/*COMPLICATIONS/*DIAGNOSIS/  MICROBIOLOGY  Cerebrospinal
       Fluid/*MICROBIOLOGY  Human  *HIV-1  Leukocyte Count  Nervous System
       Diseases/COMPLICATIONS/DIAGNOSIS/MICROBIOLOGY  Prospective Studies
       Support, U.S. Gov't, P.H.S.  T-Lymphocyte Subsets  T4 Lymphocytes
       Virology/METHODS  Virus Diseases/*COMPLICATIONS/*DIAGNOSIS/MICROBIOLOGY
       CLINICAL TRIAL  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).